甘露糖结合蛋白识别胶质瘤细胞：通过凝集素途径对胶质瘤细胞补体激活的体外分析
Mannose-binding protein recognizes glioma cells: in vitro analysis of complement activation on glioma cells via the lectin pathway.
作者信息
Fujita T, Taira S, Kodama N, Matsushita M, Fujita T
机构信息
Department of Neurosurgery, Fukushima Medical School.
出版信息
Jpn J Cancer Res. 1995 Feb;86(2):187-92. doi: 10.1111/j.1349-7006.1995.tb03038.x.
Abstract
The lectin pathway is a novel pathway for activation of the complement cascade, which is initiated by the binding of mannose-binding protein (MBP) to its carbohydrate ligands. We investigated whether the complement system was activated in vitro by glioma cells through this pathway to the C3 level. MBP was found to bind to all six glioma cell lines tested by using flow cytometric analysis. Binding of a complex of MBP-associated serine protease and MBP was observed in two of the cell lines examined, thereby resulting in C4 consumption. Activation of C3 was hemolytically evaluated in these two lines. C3 consumption was also observed in one. Based on these results, it is likely that recognition by MBP followed by complement activation occurs in certain glioma cell lines.
摘要
凝集素途径是补体级联激活的一条新途径，它由甘露糖结合蛋白（MBP）与其碳水化合物配体结合启动。我们研究了胶质瘤细胞是否能在体外通过该途径将补体系统激活至C3水平。通过流式细胞术分析发现，MBP能与所有六种受试胶质瘤细胞系结合。在所检测的两个细胞系中观察到了MBP相关丝氨酸蛋白酶与MBP复合物的结合，从而导致C4消耗。在这两个细胞系中对C3激活进行了溶血评估。在其中一个细胞系中也观察到了C3消耗。基于这些结果，在某些胶质瘤细胞系中很可能发生了MBP识别继而补体激活的情况。
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