Hoffman R K
Appl Microbiol. 1968 Apr;16(4):641-4. doi: 10.1128/am.16.4.641-644.1968.
The activity of a vapor-phase disinfectant is usually expressed in terms of the atmospheric relative humidity (RH). This study shows that, in beta-propiolactone (BPL) vapor disinfection, the important factor is really the moisture content and location of water in the cell, and not necessarily the atmospheric RH. Previous studies revealed that only about 50% of the bacterial spores equilibrated to 45% RH were killed when exposed to the same RH to BPL vapor. On the other hand, all the spores equilibrated to and then exposed at 75% RH to BPL were readily killed. The present study shows that spores equilibrated to 98% RH are readily killed by BPL at 45% RH, but only 99% of the spores equilibrated to 75% RH are killed by BPL at 45% RH. Also, in order to be killed, desiccated spores must be exposed to BPL at higher humidities than would be required if the spores had not been previously desiccated.
气相消毒剂的活性通常以大气相对湿度（RH）来表示。本研究表明，在β-丙内酯（BPL）蒸汽消毒中，重要因素实际上是细胞内水分的含量和位置，而不一定是大气相对湿度。先前的研究表明，当暴露于与BPL蒸汽相同的相对湿度时，只有约50%平衡至45%相对湿度的细菌孢子被杀死。另一方面，所有平衡至75%相对湿度然后暴露于BPL的孢子都很容易被杀死。本研究表明，平衡至98%相对湿度的孢子在45%相对湿度下很容易被BPL杀死，但在45%相对湿度下，只有99%平衡至75%相对湿度的孢子被BPL杀死。此外，为了被杀死，干燥的孢子必须在比未预先干燥的孢子更高的湿度下暴露于BPL。
