人中性粒细胞杀菌氧化酶系统中细胞色素b成分的亚细胞分布及某些特性
The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils.
作者信息
Segal A W, Jones O T
出版信息
Biochem J. 1979 Jul 15;182(1):181-8. doi: 10.1042/bj1820181.
Abstract
A novel cytochrome b has recently been described in human neutrophils. The subcellular distribution of this cytochrome was investigated by analytical fractionation on continuous sucrose gradients and it was found to have a dual localization, the major component having a similar distribution to the plasma-membrane marker [3H]concanavalin A, and a denser peak located with the specific granules. The two components were separated on discontinuous gradients and studied independently.
摘要
最近在人类中性粒细胞中发现了一种新型细胞色素b。通过在连续蔗糖梯度上进行分析分级分离研究了这种细胞色素的亚细胞分布，发现它具有双重定位，主要成分的分布与质膜标记物[3H]伴刀豆球蛋白A相似，还有一个密度更大的峰与特异性颗粒位于一起。这两种成分在不连续梯度上分离并分别进行了研究。
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