Skehan P, Friedman S J
Cell Biol Int Rep. 1979 Sep;3(6):535-42. doi: 10.1016/0309-1651(79)90089-4.
Generation time analysis by time lapse cinematography is an important method for investigating cellular senescence in culture, but its interpretation is complicated by several types of bias and artifact, including small sample size, cut-off bias, changes in global growth rate, and phase of the population growth cycle. When these factors are considered, interpretation of the data base used by previous investigators changes considerably, and does not reveal any differences in growth behavior between middle and late passage WI-38 cells. Nor does it support the transition probability theory either of cell cycle transit or of culture senescence.
通过延时摄影进行世代时间分析是研究培养细胞衰老的一种重要方法，但其解释因几种偏差和假象而变得复杂，包括样本量小、截断偏差、总体生长速率变化以及群体生长周期的阶段。当考虑这些因素时，先前研究者所使用的数据库的解释会发生很大变化，并且未揭示中期和后期传代的WI - 38细胞在生长行为上的任何差异。它也不支持细胞周期过渡或培养衰老的转变概率理论。
