Pleban P A, Pearson K H
Clin Chem. 1979 Nov;25(11):1915-8.
We describe methods for determination of manganese in whole blood and serum with Zeeman-effect flameless atomic absorption spectroscopy. These analyses are performed on a twofold or fourfold dilution of the specimen in Triton X-100, 1 g/L. No predigestion or extraction procedures are required. The method of standard additions was used for quantitation. Within-run coefficients of variation for whole-blood manganese were 7.0 and 5.5% for 2.29 and 5.67 micrograms/L, respectively. For determination of serum manganese, coefficients of variation were 10.3 and 5.3% for 0.97 and 3.01 micrograms/L, respectively. Manganese detection limits for the assays were 3.0 pg. Whole-blood manganese concentrations, determined for 60 subjects, yielded a mean (+/- SD) of 9.03 (+/- 2.25) micrograms/L. Mean serum manganese concentration, determined for 20 subjects, was 1.82 (+/- 0.64) microgram/L. No correlation was found between blood manganese concentrations and age, sex, or smoking status.
我们描述了采用塞曼效应无火焰原子吸收光谱法测定全血和血清中锰的方法。这些分析是在将标本用1 g/L的 Triton X-100进行两倍或四倍稀释后进行的。无需预消化或萃取程序。采用标准加入法进行定量。全血锰的批内变异系数在锰含量为2.29和5.67微克/升时分别为7.0%和5.5%。对于血清锰的测定，在锰含量为0.97和3.01微克/升时，变异系数分别为10.3%和5.3%。该测定方法的锰检测限为3.0皮克。对60名受试者测定的全血锰浓度，其平均值（±标准差）为9.03（±2.25）微克/升。对20名受试者测定的血清锰平均浓度为1.82（±0.64）微克/升。未发现血锰浓度与年龄、性别或吸烟状况之间存在相关性。
