链霉素依赖型霍乱弧菌的体外和体内研究。
In vitro and in vivo studies of streptomycin-dependent cholera vibrios.
作者信息
Felsenfeld O, Stegherr-Barrios A, Aldová E, Holmes J, Parrott M W
出版信息
Appl Microbiol. 1970 Mar;19(3):463-9. doi: 10.1128/am.19.3.463-469.1970.
Abstract
Streptomycin-dependent cholera vibrio strains were derived from Inaba, Ogawa, and NAG vibrios by the method of Mel. These phenotypes grew more slowly and attacked fermentable substances after a longer period of time than the streptomycin-sensitive parent strains. Rabbits injected with streptomycin-sensitive strains and their streptomycin-dependent forms showed homologous agglutinin production. Patas monkeys fed with 10(9) streptomycin-dependent strains shed them for 1 to 2 days without ill effect, whereas the same number of streptomycin-independent organisms caused disease. The possibility of the application of multiple doses of streptomycin-dependent organisms in oral immunization against cholera was considered.
摘要
链霉素依赖型霍乱弧菌菌株是通过梅尔的方法从稻叶型、小川型和NAG弧菌中获得的。这些表型的生长比链霉素敏感的亲本菌株更慢，并且在更长时间后才攻击可发酵物质。注射链霉素敏感菌株及其链霉素依赖型菌株的兔子产生了同源凝集素。用10⁹个链霉素依赖型菌株喂养的狨猴排出这些菌株1至2天，没有不良影响，而相同数量的非链霉素依赖型菌株则会引发疾病。人们考虑了在霍乱口服免疫中应用多剂量链霉素依赖型菌株的可能性。
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