Wynne E S, Henrikson D M, Daye G T
Appl Microbiol. 1970 May;19(5):731-3. doi: 10.1128/am.19.5.731-733.1970.
A live oral vaccine prepared from an Escherichia coli-Shigella flexneri 4b hybrid was administered by gavage to Macaca mulatta. In the three main studies involving 160 monkeys, three doses were used, generally consisting of 5 x 10(9) to 60 x 10(9) cells per dose, with an interval between doses of 6, 7, or 14 days. Rectal swab cultures at the time of the last vaccine dose, and 14 to 58 days later, revealed that the hybrid persisted in the intestinal tract for at least 7 days in 16%, and for at least 21 days in 8%, of the monkeys. Our findings are comparable to those of Formal et al. for shedding of an E. coli-S. flexneri 2a hybrid. Protection studies with the E. coli-S. flexneri 4b hybrid are indicated.
一种由大肠杆菌-福氏志贺菌4b杂交菌株制备的口服活疫苗经灌胃给予恒河猴。在涉及160只猴子的三项主要研究中，使用了三剂疫苗，通常每剂由5×10⁹至60×10⁹个细胞组成，剂间间隔为6、7或14天。在最后一剂疫苗接种时以及14至58天后采集的直肠拭子培养物显示，该杂交菌株在16%的猴子肠道中持续存在至少7天，在8%的猴子肠道中持续存在至少21天。我们的研究结果与Formal等人关于大肠杆菌-福氏志贺菌2a杂交菌株排出情况的研究结果相当。表明需要对大肠杆菌-福氏志贺菌4b杂交菌株进行保护性研究。
