Bidawid S, Chou S, Ng C W, Perry E, Kasatiya S
Ottawa Public Health Laboratory, Ontario, Canada.
Antonie Van Leeuwenhoek. 1989;55(2):123-31. doi: 10.1007/BF00404752.
Fatty acid profiles of purified elementary bodies of Chlamydia trachomatis (CT) serotypes D, G and L3 were investigated by gas liquid chromatography (GLC) utilizing three fused silica capillary columns of different polarities. CT serotype C and C. psittaci (CP) strain DD34 were investigated using one column only due to the lack of adequate quantities of purified material. Significantly similar fatty acid profiles were observed in the serotypes examined. However, based on the percentage ratio of 13-methyl tetradecanoate (i-15:0) to 12-methyl tetradecanoate (a-15:0), serotypes D and L3, with ratios of 0.18 and 0.19, respectively, could be differentiated from serotypes C and G with ratios of 1.3 and 1.5, respectively. CP demonstrated a ratio of 0.4, thus differentiating it from the CT serotypes examined. Fatty acids i-15:0 and a-15:0 were absent in uninfected McCoy cells. Results were significantly comparable in all three capillary columns. This study suggests that GLC could be used for identification and differentiation of Chlamydia serotypes.
利用三根不同极性的熔融石英毛细管柱，通过气相色谱法（GLC）研究了沙眼衣原体（CT）血清型D、G和L3纯化原体的脂肪酸谱。由于缺乏足够数量的纯化材料，仅使用一根柱子对CT血清型C和鹦鹉热衣原体（CP）菌株DD34进行了研究。在所检测的血清型中观察到显著相似的脂肪酸谱。然而，根据13 - 甲基十四烷酸（i - 15:0）与12 - 甲基十四烷酸（a - 15:0）的百分比比率，血清型D和L3的比率分别为0.18和0.19，可与血清型C和G区分开来，血清型C和G的比率分别为1.3和1.5。CP的比率为0.4，因此将其与所检测的CT血清型区分开来。未感染的 McCoy细胞中不存在脂肪酸i - 15:0和a - 15:0。在所有三根毛细管柱中结果具有显著可比性。本研究表明，气相色谱法可用于衣原体血清型的鉴定和区分。
