Hardy D A, Knight S, Ling N R
Immunology. 1970 Aug;19(2):329-42.
Human peripheral lymphocytes from all donors tested were mitotically activated when cultured in the presence of X-irradiated cells from continuously cultivated lymphoid cell lines (LCL). X-irradiated cells from LCL originating from normal individuals were effective as well as those of Burkitt's tumour origin and cells from LCL reported to be free of herpes-like virus were as effective as those reported to contain virus. The activation was of much greater intensity than the normal mixed lymphocyte reaction but occurred over a similar culture period. The reaction occurred only under conditions permitting direct cell contact between the two populations of cells. Most stimulation was obtained with viable X-irradiated LCL cells. Disrupted LCL cells were ineffective.
在来自持续培养的淋巴样细胞系(LCL)的经X射线照射的细胞存在的情况下培养时,所有检测供体的人外周淋巴细胞都会发生有丝分裂激活。来自正常个体的LCL的经X射线照射的细胞以及伯基特肿瘤来源的细胞是有效的,并且据报道不含疱疹样病毒的LCL细胞与据报道含有病毒的细胞一样有效。这种激活的强度比正常混合淋巴细胞反应大得多,但发生在相似的培养期内。该反应仅在允许两种细胞群体直接细胞接触的条件下发生。用活的经X射线照射的LCL细胞获得的刺激最大。破碎的LCL细胞无效。