Monath T P, Lindsey H S, Nuckolls J G, Chappell W A, Henderson B E
Appl Microbiol. 1970 Nov;20(5):748-53. doi: 10.1128/am.20.5.748-753.1970.
Human sera were treated with kaolin, acetone, and dextran sulfate to determine the best method for removing nonspecific hemagglutination inhibitors. Results indicated that on surveys for group A, group B, and some group C arbovirus HI antibodies, dextran sulfate treatment of sera could be used effectively. This method, however, has limited usefulness for detecting HI antibody for a number of arboviruses, particularly some members of the Bunyamwera supergroup since nonspecific inhibitors for these antigens were not completely removed. HI antibodies in sera drawn early after dengue and Venezuelan equine encephalitis infection were detected more readily after dextran sulfate treatment than after kaolin treatment. Kaolin, but not dextran sulfate, was shown to remove antibody from IgM fractions of sera.
用人血清与高岭土、丙酮和硫酸葡聚糖进行处理,以确定去除非特异性血凝抑制物的最佳方法。结果表明,在检测A组、B组和部分C组虫媒病毒血凝抑制(HI)抗体时,血清的硫酸葡聚糖处理可有效使用。然而,该方法在检测多种虫媒病毒的HI抗体时用途有限,特别是布尼亚姆韦拉超群的一些成员,因为这些抗原的非特异性抑制剂未被完全去除。登革热和委内瑞拉马脑炎感染后早期采集的血清中的HI抗体,经硫酸葡聚糖处理后比经高岭土处理后更容易检测到。结果显示,高岭土而非硫酸葡聚糖可从血清的IgM组分中去除抗体。