Northrop J, Slepecky R A
Science. 1967 Feb 17;155(3764):838-9. doi: 10.1126/science.155.3764.838.
When spores of Bacillus subtilis strain Marburg are heated (90 degrees to 100 degrees C) in a vacuum for 9 to 12 hours and then plated, numerous mutants are obtained, and very few spores are killed. Disproportionately large numbers of these mutants exhibit abnormal sporulation.
当枯草芽孢杆菌马尔堡菌株的孢子在真空中加热（90摄氏度至100摄氏度）9至12小时，然后进行平板接种时，会获得大量突变体，并且很少有孢子被杀死。这些突变体中数量不成比例地多的个体表现出异常的孢子形成。
