Davis W W, Stout T R
Appl Microbiol. 1971 Oct;22(4):666-70. doi: 10.1128/am.22.4.666-670.1971.
A detailed disc plate procedure is introduced for assay of antibiotics. The procedure is based on a previous study by the authors and deviates from conventional procedures in several respects: selected plastic petri dishes are employed; critical temperature control is simply provided at all stages of the test with refrigeration of the plates never used; all dilution is done with displacement microburettes; six pads (6.3 mm diameter) per dish are employed, all filled with the same unknown or reference solution; the sequence of all plates handled on 1 day is made a part of the protocol which allows accounting for the influence of the order of pouring and setting the plates; external reference plates are set at specified locations in the sequence; and, by averaging the diameters of all zones on a plate, most of the consequence of wedge shape of agar in plates, which is common and almost unavoidable, is removed. The present method is economical, uses simple facilities, and provides good accuracy of test results. Bacillus subtilis was most commonly employed, but other organisms may be employed in the present procedure.
介绍了一种用于抗生素检测的详细平板法。该方法基于作者之前的一项研究，在几个方面与传统方法不同：使用选定的塑料培养皿；在测试的所有阶段仅通过对平板进行冷藏来进行关键温度控制，从不使用未冷藏的平板；所有稀释均使用置换式微量滴定管进行；每个培养皿使用六个垫片（直径6.3毫米），所有垫片都填充相同的未知或参考溶液；将一天内处理的所有平板的顺序作为方案的一部分，这有助于考虑倾注和放置平板顺序的影响；外部参考平板按顺序设置在指定位置；并且，通过平均平板上所有抑菌圈的直径，消除了平板中琼脂楔形形状（这是常见且几乎不可避免的）的大部分影响。本方法经济实惠，使用简单的设备，并能提供良好的测试结果准确性。最常使用枯草芽孢杆菌，但本方法也可使用其他微生物。
