Kabay A
Appl Microbiol. 1971 Nov;22(5):752-5. doi: 10.1128/am.22.5.752-755.1971.
A rapid quantitative assay of 14 antibiotics and 6 chemical preservatives by the cylinder-plate diffusion method is described, in which Bacillus stearothermophilus ATCC 12980 was used as the test organism. After 4 hr of incubation at 65 C, the zones of inhibition can be read off easily with the naked eye. The 4-hr assay was compared under identical conditions with the conventional 16- to 18-hr agar diffusion assay, i.e., by using the test organisms and media proposed by the Code of Federal Regulations, and no difference in accuracy between the two methods was detected. In both cases, the coefficient of variation for replicate tests was less than 2%. The test strain is highly sensitive to penicillins, cephalosporins, and bacitracin and is consequently also particularly suitable for the determination of low-level concentrations of these substances in aqueous solutions and in urine.
本文描述了一种用圆筒平板扩散法对14种抗生素和6种化学防腐剂进行快速定量测定的方法，其中嗜热脂肪芽孢杆菌ATCC 12980被用作测试菌株。在65℃下培养4小时后，抑菌圈可轻易用肉眼读出。在相同条件下，将4小时测定法与传统的16至18小时琼脂扩散测定法进行了比较，即使用联邦法规法典中提出的测试菌株和培养基，未检测到两种方法在准确性上的差异。在这两种情况下，重复测试的变异系数均小于2%。该测试菌株对青霉素、头孢菌素和杆菌肽高度敏感，因此也特别适用于测定水溶液和尿液中这些物质的低浓度。
