Ikeda M, Hosotani T, Kurimoto K, Mori T, Ueda T, Kotake Y, Sakakibara B
J Nutr Sci Vitaminol (Tokyo). 1979;25(3):131-9. doi: 10.3177/jnsv.25.131.
The differences of the metabolism related to vitamin B6-dependent enzymes were investigated using germ-free and conventional rats. There was a significant difference in the boyd weight gain between vitamin B6-deficient germ-free and conventional rats after about 30 days of the experiment, and the body weight gain was much less in the deficient germ-free rats than in the deficient conventional ones. Urinary excretion of xanthurenic acid was higher in the deficient germ-free rats than in the deficient conventional ones after 18 days. There was a significant difference in the activities of kynurenine aminotransferase in mitochondrial fractions of germ-free rats, but not in mitochondrial fractions of conventional ones. The activities of aspartate and alanine aminotransferases, with or without pyridoxal phosphate, significantly decreased in the deficient germ-free rats, but not in the deficient conventional ones. These findings indicate that the degree of vitamin B6 deficiency was more severe in the deficient germ-free rats than in the deficient conventional ones, and also suggest that intestinal microflora may have some effects on vitamin B6-deficient conventional rats.
使用无菌大鼠和普通大鼠研究了与维生素B6依赖性酶相关的代谢差异。实验约30天后，维生素B6缺乏的无菌大鼠和普通大鼠在体重增加方面存在显著差异，缺乏维生素B6的无菌大鼠体重增加比缺乏维生素B6的普通大鼠少得多。18天后，缺乏维生素B6的无菌大鼠尿中黄尿酸排泄量高于缺乏维生素B6的普通大鼠。无菌大鼠线粒体部分中犬尿氨酸转氨酶的活性存在显著差异，而普通大鼠线粒体部分中则无显著差异。无论有无磷酸吡哆醛，缺乏维生素B6的无菌大鼠中天冬氨酸转氨酶和丙氨酸转氨酶的活性均显著降低，而缺乏维生素B6的普通大鼠中则未降低。这些发现表明，缺乏维生素B6的无菌大鼠中维生素B6缺乏的程度比缺乏维生素B6的普通大鼠更严重，也表明肠道微生物群可能对缺乏维生素B6的普通大鼠有一些影响。
