Su K L, Schmid H H
J Lipid Res. 1972 Jul;13(4):452-7.
cis-9-[1-(14)C]Octadecenol, cis,cis-9,12-[1-(14)C]octadecadienol, and cis,cis,cis-9,12,15-[1-(14)C]octadecatrienol were administered intracerebrally to 18-day-old rats. Incorporation of radioactivity into the constituent alkyl, alk-1-enyl, and acyl moieties of the ethanolamine phosphatides of brain was determined after 3, 6, 24, and 48 hr. Incorporation of radioactivity from each precursor proceeded at approximately the same rate leading to mono-, di-, and triunsaturated alkyl and alk-1-enyl glycerols. In addition, the labeled alcohols were found to be oxidized to the corresponding fatty acids which were incorporated into acyl groups; radioactivity derived from di- and triunsaturated alcohols was found mainly in acyl moieties produced through chain elongation and desaturation reactions of di- and triunsaturated fatty acids.
将顺式-9-[1-(14)C]十八碳烯醇、顺式,顺式-9,12-[1-(14)C]十八碳二烯醇和顺式,顺式,顺式-9,12,15-[1-(14)C]十八碳三烯醇脑内注射给18日龄大鼠。在3、6、24和48小时后，测定放射性掺入脑乙醇胺磷脂的组成烷基、烯丙基和酰基部分的情况。来自每种前体的放射性掺入以大致相同的速率进行，生成单不饱和、二不饱和和三不饱和烷基和烯丙基甘油。此外，发现标记的醇被氧化为相应的脂肪酸，这些脂肪酸被掺入酰基中；来自二不饱和和三不饱和醇的放射性主要存在于通过二不饱和和三不饱和脂肪酸的链延长和去饱和反应产生的酰基部分。
