Schmid H H, Takahashi T
J Lipid Res. 1970 Sep;11(5):412-9.
Palmitic acid-1-(14)C and hexadecanol-1-(14)C were administered intracerebrally to 18-day-old rats. Incorporation of radioactivity into the constituent alkyl, alk-1-enyl, and 1-acyl moieties, as well as into the 2-acyl moieties, of the ethanolamine phosphatides of brain was determined after 1, 2, 3, 6, and 22 hr. Incorporation of radioactivity from hexadecanol into both alkyl ethers and alk-1-enyl ethers proceeded at a rate more than 10 times higher than from palmitic acid. Hexadecanol was rapidly oxidized to fatty acids which were incorporated into the acyl moieties of the ethanolamine phosphatides. When palmitic acid was used as a precursor, labeled long-chain alcohols could be isolated from the lipid extract. As labeled long-chain aldehydes could not be detected in any of the lipid extracts, alcohols appear to be key intermediates for the biosynthesis of both alkyl and alk-1-enyl glycerophosphatides.
将棕榈酸 -1-(¹⁴)C 和十六醇 -1-(¹⁴)C 脑内注射给 18 日龄大鼠。在 1、2、3、6 和 22 小时后，测定放射性掺入脑乙醇胺磷脂的组成烷基、alk -1 -烯基和 1 -酰基部分以及 2 -酰基部分的情况。十六醇的放射性掺入烷基醚和 alk -1 -烯基醚的速率比棕榈酸高出 10 倍以上。十六醇迅速氧化为脂肪酸，这些脂肪酸被掺入乙醇胺磷脂的酰基部分。当使用棕榈酸作为前体时，可从脂质提取物中分离出标记的长链醇。由于在任何脂质提取物中均未检测到标记的长链醛，因此醇似乎是烷基和 alk -1 -烯基甘油磷脂生物合成的关键中间体。
