Lewensohn R, Ringborg U
Blood. 1979 Dec;54(6):1320-9.
A technique has been developed for handling human bone marrow cells intended for the examination of DNA repair synthesis. DNA-repair synthesis, induced by melphalan and nitrogen mustard, was measured as the incorporation of 3H-thymidine, registered by autoradiography as unscheduled DNA synthesis (UDS). Comparison of various cell populations disclosed considerable differences in their UDS level, this generally being greatest for the blast populations. During maturation of both myelopoietic and erythropoietic cells, there was a decrease in the UDS level, which was lowest for the end-cell stage. The lymphocytes and monocytes differed considerably in their capacity for UDS. The developed technique would appear to offer an opportunity for determining the capacity for DNA-repair synthesis in malignant bone marrow cells, a factor that has been linked to sensitivity to alkylating agents.
已开发出一种用于处理旨在检测DNA修复合成的人类骨髓细胞的技术。由美法仑和氮芥诱导的DNA修复合成，通过3H-胸腺嘧啶核苷的掺入来测量，通过放射自显影记录为非预定DNA合成（UDS）。对各种细胞群体的比较揭示了它们的UDS水平存在相当大的差异，这通常在原始细胞群体中最为显著。在骨髓细胞和红细胞的成熟过程中，UDS水平下降，在终末细胞阶段最低。淋巴细胞和单核细胞在其UDS能力上有很大差异。所开发的技术似乎为确定恶性骨髓细胞中的DNA修复合成能力提供了一个机会，这一因素与对烷化剂的敏感性有关。
