Sazama K, Robertson E A, Chesler R A
Clin Chem. 1979 Dec;25(12):2038-9.
We obtained -68 pairs of simultaneously drawn serum and fluoride-oxalate plasma samples from patients and analyzed them by a continuous-flow (AutoAnalyzer II) glucose oxidase method. Glucose concentrations ranged from 370 to 3530 mg/L. Glucose concentrations for samples obtained in the fluoride-oxalate preservative averaged 42 +/- 35 mg/L (mean +/- SD) higher than serum. The magnitude of this difference was independent of glucose concentration. Linear-regression analysis of 270 pairs for which the time from collection to separation was recorded indicated that the difference between serum and plasma increased by 0.32 mg/L per minute of delay over a time span of 15 to 295 min. These differences are smaller than those described in standard textbooks. We conclude that, with the specimen-handling process used in our hospital, serum glucose determinations are clinically acceptable.
我们从患者身上获取了68对同时采集的血清和氟化物 - 草酸盐血浆样本，并通过连续流动（自动分析仪II型）葡萄糖氧化酶法对其进行分析。葡萄糖浓度范围为370至3530mg/L。在氟化物 - 草酸盐防腐剂中获得的样本的葡萄糖浓度平均比血清高42±35mg/L（平均值±标准差）。这种差异的大小与葡萄糖浓度无关。对记录了从采集到分离时间的270对样本进行线性回归分析表明，在15至295分钟的时间跨度内，血清和血浆之间的差异每延迟一分钟增加0.32mg/L。这些差异比标准教科书中描述的要小。我们得出结论，就我们医院使用的标本处理过程而言，血清葡萄糖测定在临床上是可接受的。
