Lloyd J B
Biochem J. 1971 Jan;121(2):245-8. doi: 10.1042/bj1210245.
The latency of nitrocatechol sulphatase activity was measured in rat liver lysosomes before and after preincubation in 0.25m solutions of five amino acids, three dipeptides and a tripeptide. Rates of increase in ;free' activity were taken as an indication of rates of solute penetration into lysosomes and were correlated with the structure of each molecule studied. In general permeability was greater in solutions of pH7 than of pH5 or 6, and dipeptides entered more rapidly than amino acids or triglycine. The conclusions are in good agreement with those obtained by other methods.
在五种氨基酸、三种二肽和一种三肽的0.25m溶液中预孵育前后，测定大鼠肝脏溶酶体中硝基儿茶酚硫酸酯酶活性的潜伏期。“游离”活性的增加速率被用作溶质渗透到溶酶体中的速率指标，并与所研究的每个分子的结构相关。一般来说，pH7溶液中的通透性比pH5或6溶液中的通透性大，二肽比氨基酸或三甘肽进入得更快。这些结论与通过其他方法获得的结论非常一致。
