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电子显微镜放射自显影中的定量与分辨率

Quantitation and resolution in electron microscope radioautography.

作者信息

Nadler N J

出版信息

J Histochem Cytochem. 1979 Nov;27(11):1531-3. doi: 10.1177/27.11.512340.

DOI:10.1177/27.11.512340
PMID:512340
Abstract

Electron microscopy can resolve structures with accuracy of the order of 0.2-0.3 nm, but the radioautographic technique is able to locate radioactive label with much less certainty, at best 50 nm. This difference in resolution is what creates the problem of quantitation in electron microscope radioautographs.

摘要

电子显微镜能够分辨出精度约为0.2至0.3纳米的结构，但放射自显影技术确定放射性标记位置的准确性要低得多，最多只能达到50纳米。分辨率上的这种差异正是造成电子显微镜放射自显影片定量问题的原因。

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