Kaneta S, Endo Y, Fujihira E, Mitsuya M
J Immunol Methods. 1979;31(1-2):151-8. doi: 10.1016/0022-1759(79)90295-3.
Following the i.p. challenge of a shocking dose of BSA from 9 days up to 133 days after the s.c. injection of BSA in CFA, fatal anaphylaxis was induced regularly in female ICR mice that had been given the immunizing antigen when 8 weeks old. These immunized mice provided an antiserum to BSA that had the capacity to transfer fatal shock to normal recipient mice at a minimum Ab-N dose of 8 microgram when the i.p. route for challenge was employed. The optimal dose-range of antigen and antibody in order to elicit fatal shock following the i.p. challenge was much broader than that obtained by i.v. injection. Age is critical in producing fatal shock in mice; a 100% fatal anaphylaxis never occurred in groups of 6- and 7-week-old recipient mice although those at 8 weeks and older were sufficiently sensitized by the amounts of antibody given.
在弗氏完全佐剂中皮下注射牛血清白蛋白（BSA）后9天至133天，对雌性ICR小鼠腹腔注射大剂量BSA进行激发，在8周龄时接受免疫抗原的雌性ICR小鼠中经常诱发致命性过敏反应。这些免疫小鼠产生了抗BSA血清，当采用腹腔注射途径进行激发时，以最低8微克抗体氮（Ab-N）剂量就能将致命性休克转移给正常受体小鼠。腹腔注射激发后引发致命性休克的抗原和抗体的最佳剂量范围比静脉注射获得的范围要宽得多。年龄对小鼠产生致命性休克至关重要；6周龄和7周龄的受体小鼠组中从未发生100%致命性过敏反应，尽管8周龄及以上的小鼠通过给予的抗体量已充分致敏。
