红细胞对商陆有丝分裂原促有丝分裂特性的灭活作用。
Inactivation of the mitogenic property of pokeweed mitogen by erythrocytes.
作者信息
Ohno R, Kodera Y, Ezaki K, Tanimoto M, Iwamoto K, Yamada K
出版信息
J Immunol Methods. 1979;31(1-2):65-9. doi: 10.1016/0022-1759(79)90286-2.
In whole blood culture, pokeweed mitogen (PWM) is unable to stimulate human lymphocytes. This is because the mitogenic property of PWM is inactivated by erythrocytes, presumably due to absorption. The inactivation was observed with as few as 5 x 10(6)/ml of human erythrocytes. Therefore, when PWM is used to study the functions of human lymphocytes, especially of suppressor T lymphocytes, erythrocytes should be removed from lymphocyte preparations for accurate analysis.
在全血培养中，商陆有丝分裂原（PWM）无法刺激人类淋巴细胞。这是因为PWM的促有丝分裂特性被红细胞灭活，推测是由于吸附作用。在每毫升仅含5×10⁶个红细胞时就能观察到这种灭活现象。因此，当使用PWM来研究人类淋巴细胞尤其是抑制性T淋巴细胞的功能时，为了进行准确分析，应从淋巴细胞制剂中去除红细胞。
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