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能否使用全血而非分离出的低密度细胞来检测有丝分裂原反应?

Can whole blood be used instead of separated low density cells for examination of the mitogen response?

作者信息

Nissen C, Albrecht R, Corneo M, Bruun D, Speck B

出版信息

Blut. 1981 Jul;43(1):41-6. doi: 10.1007/BF00319930.

DOI:10.1007/BF00319930
PMID:7260402
Abstract

The response of whole blood and separated low density cells to 3 mitogenic lectins [Phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin (Con-A)] was tested in 133 normal individual of all ages, including cord blood. The results obtained with the two methods correlated poorly (r = 0.16 for PHA; 0.47 for PWM and 0.42 for Con-A). Thus, the two methods measure different things and results are not comparable.

摘要

在包括脐血在内的133名各年龄段的正常个体中,检测了全血和分离出的低密度细胞对3种促有丝分裂凝集素[植物血凝素(PHA)、商陆有丝分裂原(PWM)和刀豆球蛋白A(Con-A)]的反应。两种方法得到的结果相关性较差(PHA的r = 0.16;PWM的r = 0.47;Con-A的r = 0.42)。因此,这两种方法测量的是不同的东西,结果不可比。

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本文引用的文献

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Lymphocyte transformation during pregnancy: an analysis using whole-blood culture.
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2
Microtechnique for quantitative evaluation of in vitro lymphocyte transformation.体外淋巴细胞转化定量评估的显微技术
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Lymphocyte transformation using whole blood cultures: an analysis of responses.使用全血培养进行淋巴细胞转化:反应分析
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Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.从人血中分离单核细胞和粒细胞。通过一次离心分离单核细胞,通过离心和1g沉降相结合的方法分离粒细胞。
Scand J Clin Lab Invest Suppl. 1968;97:77-89.
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[Lymphocyte stimulation in various age groups].[不同年龄组的淋巴细胞刺激]
Schweiz Med Wochenschr. 1978 Apr 15;108(15):551-5.
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An analysis of phytohemagglutinin lymphoblastogenesis in whole blood.全血中植物血凝素淋巴细胞生成的分析
Surg Gynecol Obstet. 1978 Apr;146(4):609-16.
8
An application of the whole blood microculture technique to the detection of lymphocyte transformation.全血微量培养技术在淋巴细胞转化检测中的应用。
Osaka City Med J. 1978;24(2):155-64.