LaForce F M, Brice J L, Tornabene T G
J Infect Dis. 1979 Oct;140(4):453-64. doi: 10.1093/infdis/140.4.453.
The feasibility of gas-liquid chromatographic analysis of spinal fluid from dogs with experimental meningitis and from patients with meningitis was evaluated. Normal canine and human spinal fluid contained consistent levels of palmitic and stearic acids, glucose, and trace amounts of glycerol. Pneumococcal meningitis in dogs and humans was characterized by a complex fatty-acid pattern in spinal fluid similar to that seen in pneumococcal cells. Spinal fluid from dogs with Haemophilus influenzae meningitis yielded several saturated fatty acids as well as rhamnose, xylose, and glucosamine; four of five spinal fluid specimens from patients with H. influenzae meningitis gave similar results. Fatty-acid profiles of spinal fluid from dogs with staphylococcal meningitis were complex, with branched-chain and straight-chain fatty acids. Spinal fluid specimens from Neisseria meningitidis-infected dogs yielded 16- and 18-carbon fatty acids and sialic acid. Gas-liquid chromatographic analysis of spinal fluid proved valuable as a tool for identifying bacterial infections and differentiating between common spinal fluid pathogens.
对患有实验性脑膜炎的犬类以及患有脑膜炎的患者的脑脊液进行气液色谱分析的可行性进行了评估。正常犬类和人类脑脊液中棕榈酸、硬脂酸、葡萄糖以及微量甘油的含量一致。犬类和人类的肺炎球菌性脑膜炎的特征在于脑脊液中存在类似于肺炎球菌细胞中的复杂脂肪酸模式。患有流感嗜血杆菌脑膜炎的犬类的脑脊液产生了几种饱和脂肪酸以及鼠李糖、木糖和氨基葡萄糖；五份来自流感嗜血杆菌脑膜炎患者的脑脊液标本中有四份得出了类似结果。患有葡萄球菌性脑膜炎的犬类的脑脊液脂肪酸谱很复杂，含有支链和直链脂肪酸。感染脑膜炎奈瑟菌的犬类的脑脊液标本产生了16碳和18碳脂肪酸以及唾液酸。脑脊液的气液色谱分析被证明是一种有价值的工具，可用于识别细菌感染并区分常见的脑脊液病原体。
