体外培养的大鼠卵黄囊对外源蛋白质的消化作用。
Digestion of an exogenous protein by rat yolk-sac cultured in vitro.
作者信息
Williams K E, Lloyd J B, Davies M, Beck F
出版信息
Biochem J. 1971 Nov;125(1):303-8. doi: 10.1042/bj1250303.
Abstract
Yolk-sacs were removed from 17.5-day pregnant rats injected 2-5h previously with (125)I-labelled bovine serum albumin. The specific activities of acid phosphatase and acid proteinase, and the specific radioactivities (trichloroacetic acid-insoluble and trichloroacetic acid-soluble) were measured in subcellular fractions prepared by homogenization and differential centrifugation. The conversion of acid-insoluble into acid-soluble radioactivity within cultured tissue was followed and the nature of the liberated products was investigated by gel chromatography. The results are consistent with the protein entering lysosomes and being digested there. The radiolabel was released chiefly as free iodotyrosine.
摘要
从17.5天龄的怀孕大鼠中取出卵黄囊，这些大鼠在2 - 5小时前注射了(125)I标记的牛血清白蛋白。通过匀浆和差速离心制备亚细胞组分，测定其中酸性磷酸酶和酸性蛋白酶的比活性以及比放射性（三氯乙酸不溶性和三氯乙酸可溶性）。追踪培养组织中酸性不溶性放射性向酸性可溶性放射性的转化，并通过凝胶色谱研究释放产物的性质。结果表明蛋白质进入溶酶体并在那里被消化。放射性标记主要以游离碘酪氨酸的形式释放。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
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