乌昆耶米病毒含有一种RNA聚合酶。
Uukuniemi virus contains an RNA polymerase.
作者信息
Ranki M, Pettersson R F
出版信息
J Virol. 1975 Dec;16(6):1420-5. doi: 10.1128/JVI.16.6.1420-1425.1975.
Abstract
An RNA-dependent RNA polymerase activity has been found associated with Uukuniemi virions. The enzyme activity is expressed only after disrupting the virions with the nonionic detergent Triton X-100 and is absolutely dependent on Mn2+, whereas Mg2+ is not required, a finding that distinguishes this polymerase from those of other enveloped minus-strand RNA viruses. Within the range pH 7.2 to 8.5 no distinct optimum was found. The optimum temperature was between 37 and 40 C. The reaction was not inhibited by actinomycin D, rifampin, or DNase, whereas RNase was completely inhibitory. The partially RNase-resistant product consisted of rather small-sized RNA, which contained sequences complementary to Uukuniemi virus RNA as shown by hybridization to the template L, M, and S RNA species of Uukuniemi virus.
摘要
已发现乌昆耶米病毒粒子与一种依赖RNA的RNA聚合酶活性相关。该酶活性只有在用非离子去污剂曲拉通X-100破坏病毒粒子后才会表达，并且绝对依赖于Mn2+，而不需要Mg2+，这一发现将这种聚合酶与其他包膜负链RNA病毒的聚合酶区分开来。在pH 7.2至8.5范围内未发现明显的最适值。最适温度在37至40℃之间。该反应不受放线菌素D、利福平或DNA酶的抑制，而RNA酶则具有完全抑制作用。部分抗RNA酶的产物由相当小的RNA组成，通过与乌昆耶米病毒的模板L、M和S RNA种类杂交表明，其包含与乌昆耶米病毒RNA互补的序列。
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本文引用的文献
1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
ISOLATION OF SOME VIRUSES OTHER THAN TYPICAL TICK-BORNE ENCEPHALITIS VIRUSES FROM IXODES RICINUS TICKS IN FINLAND.在芬兰从蓖麻硬蜱中分离出除典型蜱传脑炎病毒之外的一些病毒。
Ann Med Exp Biol Fenn. 1964;42:109-12.
3
Antigenic characterization of tick-borne arboviruses not belonging to group B.非B组蜱传虫媒病毒的抗原特性分析
Jpn J Med Sci Biol. 1967 Dec;20 Suppl:119-29.
4
Bunyaviruses and bunyaviridae.布尼亚病毒科与布尼亚病毒
Intervirology. 1974;2(4):270-2. doi: 10.1159/000149433.
5
Passerine birds in the ecology of Uukuniemi virus.
Med Biol. 1974 Apr;52(2):98-103.
6
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Virology. 1973 Dec;56(2):608-19. doi: 10.1016/0042-6822(73)90062-7.
7
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8
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