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Mouse immunoglobulin genes: studies on the reiteration frequency of light-chain genes by hybridisation procedures.

作者信息

Rabbitts T H, Milstein C

出版信息

Eur J Biochem. 1975 Mar 3;52(1):125-33. doi: 10.1111/j.1432-1033.1975.tb03980.x.

DOI:10.1111/j.1432-1033.1975.tb03980.x
PMID:51793
Abstract

The partially purified immunoglobulin light chain messenger RNA fraction from P3K (MOPC 21) mouse myeloma tissue-culture cells has been employed in hybridisation studies. Fragments of the messenger RNA were generated by alkali hydrolysis. 6-S fragments not containing poly(A) showed the characteristic biphasic hybridisation profile seen with the intact RNA fraction. 12-S and 6-S poly(A)-containing fragments, however, showed single transitions lacking the rapidly hybridising component. Complementary DNA copies of the intact messenger RNA fraction were prepared with RNA-dependent DNA polymerase and the DNA populations fractionated on acrylamide gels. Hybridisation experiments with complementary DNA fractions up to 800 bases in length showed annealing to single (or a few) genes. A rapidly hybridising component (about 200 copies) appears in the cDNA fraction containing the largest transcripts. We conclude that the kappa constant region gene and the MOPC 21 variable region gene are present as one or a few copies in the haploid genome and that the rapidly hybridising component is not due to variable region genes.

摘要

相似文献

1
Mouse immunoglobulin genes: studies on the reiteration frequency of light-chain genes by hybridisation procedures.
Eur J Biochem. 1975 Mar 3;52(1):125-33. doi: 10.1111/j.1432-1033.1975.tb03980.x.
2
No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.未检测到编码小鼠MOPC 41免疫球蛋白轻链mRNA的基因重复。
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Estimation of the number of genes coding for the constant part of the mouse immunoglobulin kappa light chain.编码小鼠免疫球蛋白κ轻链恒定区的基因数量估计。
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引用本文的文献

1
Sequence organization of animal nuclear DNA.动物核DNA的序列组织
Hum Genet. 1980;55(1):1-18. doi: 10.1007/BF00329120.
2
Organization of kappa light chain genes in germ-line and somatic tissue.种系和体细胞组织中κ轻链基因的组织方式。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1106-10. doi: 10.1073/pnas.77.2.1106.
3
Size differences among immunoglobulin heavy chains from phosphorylcholine-binding proteins.来自磷酸胆碱结合蛋白的免疫球蛋白重链之间的大小差异。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2109-12. doi: 10.1073/pnas.73.6.2109.
4
Transcription in vitro of immunoglobulin kappa light chain genes in isolated mouse myeloma nuclei and chromatin.在分离的小鼠骨髓瘤细胞核和染色质中进行免疫球蛋白κ轻链基因的体外转录。
Proc Natl Acad Sci U S A. 1976 Mar;73(3):775-9. doi: 10.1073/pnas.73.3.775.
5
No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA.未检测到编码小鼠MOPC 41免疫球蛋白轻链mRNA的基因重复。
Proc Natl Acad Sci U S A. 1976 Mar;73(3):727-31. doi: 10.1073/pnas.73.3.727.
6
Reiteration frequency of immunoglobulin light chain genes: further evidence for somatic generation of antibody diversity.免疫球蛋白轻链基因的重复频率：抗体多样性体细胞产生的进一步证据。
Proc Natl Acad Sci U S A. 1976 Jan;73(1):203-7. doi: 10.1073/pnas.73.1.203.
7
Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis.通过克隆和序列分析鉴定出多个相关的免疫球蛋白可变区基因。
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3881-5. doi: 10.1073/pnas.75.8.3881.
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Use of molecular hybridization to purify and analyze albumin messenger RNA from rat liver.利用分子杂交技术从大鼠肝脏中纯化和分析白蛋白信使核糖核酸。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4346-50. doi: 10.1073/pnas.74.10.4346.
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Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.由免疫球蛋白κ轻链信使核糖核酸构建的重组DNA克隆。
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Proc Natl Acad Sci U S A. 1978 Aug;75(8):3913-7. doi: 10.1073/pnas.75.8.3913.