淋巴细胞与成纤维细胞杂交体中HL-A抗原在D98/AH-2细胞中的表达与分离

Expression and segregation of HL-A antigens in D98/AH-2 by lymphocyte and fibroblast hybrids.

作者信息

Kennett R H, Hampshire B, Bengtsson B, Bodmer W F

出版信息

Tissue Antigens. 1975 Aug;6(2):80-92. doi: 10.1111/j.1399-0039.1975.tb00619.x.

DOI:10.1111/j.1399-0039.1975.tb00619.x

Abstract

Somatic cell hybrids between D98/AH-2 and fibroblasts or peripheral blood lymphocytes were analysed for the expression of HL-A antigens. The hybrids express the antigens of the donor lymphocytes or fibroblasts as well as other HL-A specificities from D98/AH-2, a HeLa derivative on which no HL-A could be detected by direct cytotoxicity. Segregant hybrid clones could be used to determine HL-A haplotypes and define the linkage relationships between these haplotypes and the PGM3 alleles expressed in the hybrids. An analysis of the HL-A antigens on D98/AH-2 and other HeLa derivates was done using absorption and a modified cytotoxicity assay.

摘要

分析了D98/AH-2与成纤维细胞或外周血淋巴细胞之间的体细胞杂种的HL-A抗原表达情况。这些杂种表达供体淋巴细胞或成纤维细胞的抗原以及来自D98/AH-2的其他HL-A特异性，D98/AH-2是一种HeLa衍生物，通过直接细胞毒性检测未检测到HL-A。分离的杂种克隆可用于确定HL-A单倍型，并确定这些单倍型与杂种中表达的PGM3等位基因之间的连锁关系。使用吸收法和改良的细胞毒性试验对D98/AH-2和其他HeLa衍生物上的HL-A抗原进行了分析。