关于钠离子与可溶性核糖核酸相互作用的23Na核磁共振弛豫研究。
23Na nuclear magnetic resonance relaxation studies of sodium ion interaction with soluble RNA.
作者信息
James T L, Noggle J H
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):644-9. doi: 10.1073/pnas.62.3.644.
Abstract
Interactions between (23)Na(+) and soluble RNA in aqueous solution are studied with the use of (23)Na nuclear magnetic resonance. At low concentrations of NaCl, the interactions obey a simple equilibrium model with a formation constant log (K(f))(3) = 2.8 +/- 0.3. The relaxation rate of the bound sodium is found to be T(1B) (-1) = 222 +/- 19 sec(-1) compared to that of free sodium T(1F) (-1) = 17.5 sec(-1). At high NaCl concentrations, the system deviates from the model, possibly owing to aggregation of the soluble RNA.
摘要
利用²³Na核磁共振研究了水溶液中(23)Na⁺与可溶性RNA之间的相互作用。在低浓度NaCl条件下，相互作用符合一个简单的平衡模型，其形成常数log(K(f))(3) = 2.8 ± 0.3。发现结合钠的弛豫率为T(1B)(-1) = 222 ± 19 s⁻¹，而游离钠的弛豫率为T(1F)(-1) = 17.5 s⁻¹。在高NaCl浓度下，系统偏离该模型，可能是由于可溶性RNA的聚集。
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Biochem Biophys Res Commun. 1967 Sep 7;28(5):785-90. doi: 10.1016/0006-291x(67)90386-5.
2
Nuclear magnetic resonance studies of antibody-hapten interactions using a chloride ion probe.使用氯离子探针的抗体-半抗原相互作用的核磁共振研究。
Biochemistry. 1967 Feb;6(2):498-502. doi: 10.1021/bi00854a018.
3
Halide ions as probes for nuclear magnetic resonance studies of proteins. The sulfhydryl groups of hemoglobin.卤离子作为蛋白质核磁共振研究的探针。血红蛋白的巯基。
J Am Chem Soc. 1967 Jun 7;89(12):3045-50. doi: 10.1021/ja00988a044.
4
NMR evidence for complexing of Na+ in muscle, kidney, and brain, and by actomyosin. The relation of cellular complexing of Na+ to water structure and to transport kinetics.核磁共振（NMR）证据表明，在肌肉、肾脏和大脑中以及肌动球蛋白存在时，钠离子会形成络合物。钠离子的细胞络合与水结构及运输动力学之间的关系。
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5
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6
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7
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