Niikawa N, Kajii T
Humangenetik. 1975 Oct 20;30(1):83-90. doi: 10.1007/BF00273636.
A standardized Q- and acridine orange (AO)-fluorescence dual marker technique was described. It involved preservation of unstained chromosome slides in a vacuum desiccator up to 18 months, Q-staining, destaining, and treatment in Hanks' solution, pH 5.1, at 85 degrees C for 13 min, and acridine orange staining. Q-markers were found at the paracentromeric regions of chromosomes 3 and 4, the short arms and the satellites of the acrocentric chromosomes, while AO-marker spots were on the satellite-stalks of the acrocentrics. The advantage of the dual marker technique was illustrated by the determination of the origin of trisomy 22 in a spontaneous abortus.
描述了一种标准化的Q荧光和吖啶橙（AO）荧光双标记技术。该技术包括将未染色的染色体玻片在真空干燥器中保存长达18个月，进行Q染色、脱色，并在pH 5.1的汉克斯溶液中于85℃处理13分钟，然后进行吖啶橙染色。在3号和4号染色体的着丝粒旁区域、近端着丝粒染色体的短臂和随体上发现了Q标记，而AO标记点位于近端着丝粒染色体的随体柄上。通过对一例自然流产胎儿22三体起源的测定，说明了双标记技术的优势。
