Abdou N I, Richter M
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1136-43. doi: 10.1073/pnas.63.4.1136.
Normal adult rabbits were injected intravenously with peripheral leukocytes obtained from a normal human volunteer (donor A). The animals were sacrificed 24 hours later, and their bone marrow cells (primed bone marrow) or bone marrow cells obtained from a normal, unimmunized rabbit (normal bone marrow) were injected intravenously into irradiated (800 r) rabbits. The latter were immunized with peripheral leukocytes of donor A. The antisera obtained from the irradiated rabbits given primed bone marrow cells displayed minimal or no cytotoxic activity toward white cells of either donor A or an unrelated donor B. On the other hand, antisera obtained from irradiated rabbits given normal bone marrow cells displayed high titers of cytotoxic activity with respect to donor A cells only. It is concluded that rabbit bone marrow can be depleted of antigen-reactive cells directed toward white cell antigens following injection of the white cells intravenously and that these bone marrow cells are incapable of conferring antibody-forming capacity, in irradiated recipients, directed to this particular species of white cells. The relevance of these findings to the field of transplantation in general is discussed.
将从一名正常人类志愿者（供体A）获取的外周白细胞静脉注射到正常成年兔体内。24小时后处死这些动物，并将其骨髓细胞（致敏骨髓）或从正常未免疫兔获取的骨髓细胞（正常骨髓）静脉注射到经800伦琴照射的兔体内。后者用供体A的外周白细胞进行免疫。接受致敏骨髓细胞的经照射兔所获得的抗血清对供体A或无关供体B的白细胞显示出最小或无细胞毒性活性。另一方面，接受正常骨髓细胞的经照射兔所获得的抗血清仅对供体A细胞显示出高滴度的细胞毒性活性。得出的结论是，静脉注射白细胞后，兔骨髓中针对白细胞抗原的抗原反应性细胞可被清除，并且这些骨髓细胞在经照射的受体中无法赋予针对这种特定白细胞种类的抗体形成能力。讨论了这些发现与一般移植领域的相关性。
