Kaplan A S, Ben-Porat T
Proc Natl Acad Sci U S A. 1970 Jul;66(3):799-806. doi: 10.1073/pnas.66.3.799.
The envelope of pseudorabies virions can be removed by treatment with Triton X-100, leaving the nucleocapsid intact. The viral envelope removed in this fashion contains all the glycoproteins and about 40 per cent of the proteins of the virions. Analysis by polyacrylamide gel electrophoresis revealed that the envelope proteins migrate as four distinct peaks, all of which contain glycoproteins. The proteins of the nucleocapsids migrate in the gels as six distinct peaks.
伪狂犬病病毒粒子的包膜可以通过用曲拉通X-100处理而去除，使核衣壳保持完整。以这种方式去除的病毒包膜包含所有糖蛋白和病毒粒子约40%的蛋白质。聚丙烯酰胺凝胶电泳分析表明，包膜蛋白迁移形成四个不同的峰，所有这些峰都含有糖蛋白。核衣壳蛋白在凝胶中迁移形成六个不同的峰。
