Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane.

The envelope of pseudorabies virions can be removed by treatment with Triton X-100, leaving the nucleocapsid intact. The viral envelope removed in this fashion contains all the glycoproteins and about 40 per cent of the proteins of the virions. Analysis by polyacrylamide gel electrophoresis revealed that the envelope proteins migrate as four distinct peaks, all of which contain glycoproteins. The proteins of the nucleocapsids migrate in the gels as six distinct peaks.