MS2病毒RNA中从5'端到A蛋白起始密码子的前导序列。
The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA.
作者信息
De Wachter R, Vandenberghe A, Merregaert J, Contreras R, Fiers W
出版信息
Proc Natl Acad Sci U S A. 1971 Mar;68(3):585-9. doi: 10.1073/pnas.68.3.585.
RNA fragments of different chain length, each containing the 5'-terminal guanosine tetraphosphate (pppGp) of bacteriophage-MS2 RNA, have been isolated from partial ribonuclease digests of the viral RNA. The longest fragment overlaps with the ribosomalbinding site of the A-protein cistron. The base sequence has been established for the major part. The results directly confirm that the A-protein cistron is closest to the 5'-terminus. Its initiating (AUG) codon starts at position 130, being preceded by an untranslated sequence of 129 nucleotides.
已从噬菌体-MS2 RNA的部分核糖核酸酶消化产物中分离出不同链长的RNA片段，每个片段都含有病毒RNA的5'-末端四磷酸鸟苷（pppGp）。最长的片段与A蛋白顺反子的核糖体结合位点重叠。已确定了大部分的碱基序列。结果直接证实A蛋白顺反子最靠近5'-末端。其起始（AUG）密码子从第130位开始，前面有129个核苷酸的非翻译序列。
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