从人唾液中选择性去除溶菌酶的简单快速方法。
Simple and rapid procedure for the selective removal of lysozyme from human saliva.
作者信息
Germaine G R, Tellefson L M
出版信息
Infect Immun. 1979 Dec;26(3):991-5. doi: 10.1128/iai.26.3.991-995.1979.
Abstract
A simple and rapid method is described for the removal of lysozyme from human whole salivary supernatant. Saliva specimens were adsorbed with Micrococcus lysodeikticus. The saliva so treated was depleted of 95% of the lysozyme activity. Changes in total protein, lactoperoxidase, lactoferrin, immunoglobulin A, and the proportions of several anionic proteins were less than 10%. It is concluded that adsorption of saliva with M. lysodeikticus is a suitable procedure for the preparation of saliva that is selectively deficient in lysozyme.
摘要
本文描述了一种从人全唾液上清液中去除溶菌酶的简单快速方法。唾液标本用溶壁微球菌吸附。经如此处理的唾液中溶菌酶活性降低了95%。总蛋白、乳过氧化物酶、乳铁蛋白、免疫球蛋白A以及几种阴离子蛋白的比例变化小于10%。结论是，用溶壁微球菌吸附唾液是制备选择性缺乏溶菌酶的唾液的合适方法。
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