大鼠肝脏线粒体RNA聚合酶的部分纯化
Partial purification of mitochondrial RNA polymerase from rat liver.
作者信息
Reid B D, Parsons P
出版信息
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2830-4. doi: 10.1073/pnas.68.11.2830.
Abstract
Mitochondrial RNA polymerase activity from rat liver has previously been demonstrated in intact organelles. This activity has now been solubilized, partially purified, and shown to be a true polymerase, free of nuclease. The enzyme is derived from mitochondria and is not from contaminating bacteria or nuclear components. The enzyme is distinguished from its nuclear counterparts by its behavior on ammonium sulfate fractionation and lack of inhibition by alpha-amanitin. Rifamycin inhibits the crude enzyme, but only inconsistently inhibits the more purified preparation.
摘要
先前已在完整的细胞器中证实了大鼠肝脏线粒体RNA聚合酶的活性。现在，这种活性已被溶解、部分纯化，并显示为一种真正的聚合酶，不含核酸酶。该酶源自线粒体，并非来自污染的细菌或核成分。通过硫酸铵分级分离行为以及对α-鹅膏蕈碱不敏感，该酶与细胞核中的对应酶有所区别。利福霉素可抑制粗酶，但对纯化程度更高的制剂仅偶尔有抑制作用。
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