Dillard C J, Tappel A L
Lipids. 1979 Dec;14(12):989-95. doi: 10.1007/BF02533435.
A study was undertaken to determine whether respiratory hexanal and acetone as well as pentane and ethane could be measured as potential indices of lipid peroxidation in vivo. The tests of induction of lipid peroxidation in rats included injection of iron-dextran and the vitamin E deficiency status. Injection of 460 mg of iron/100 g body wt over a 28-day period increased pentane and ethane production 4- and 6-fold, respectively. Hexanal production was increased 7-fold after injection of 60 mg of iron/100 g body wt, and then it fell back to the preinjection level in spite of continued injection of iron-dextran. Acetone production was lower in iron-injected rats than in controls, and it was ca. 10-fold higher in fasted vitamin E-deficient rats than in vitamin E-supplemented rats, being ca 48 and 5 nmol/100 g/min, respectively. It was observed that halomethane injection did not increase hexanal production, while acetone and pentane production were increased. Pentane and hexanal, but not acetone, were found to arise from decomposition of linoleic acid hydroperoxide in vitro. It was concluded that hydrocarbon gases are better indices of lipid peroxidation than hexanal, which is enzymatically metabolized, and acetone, the production of which is dominated by factors such as altered carbohydrate metabolism.
开展了一项研究，以确定是否可以将呼吸道中的己醛、丙酮以及戊烷和乙烷作为体内脂质过氧化的潜在指标进行测量。在大鼠中诱导脂质过氧化的试验包括注射右旋糖酐铁和维生素E缺乏状态。在28天内注射460mg铁/100g体重，戊烷和乙烷的产生量分别增加了4倍和6倍。注射60mg铁/100g体重后，己醛的产生量增加了7倍，尽管继续注射右旋糖酐铁，但随后又降至注射前水平。注射铁的大鼠中丙酮的产生量低于对照组，而禁食的维生素E缺乏大鼠中的丙酮产生量比补充维生素E的大鼠高约10倍，分别约为48和5nmol/100g/分钟。观察到注射卤代甲烷不会增加己醛的产生量，而丙酮和戊烷的产生量增加。发现戊烷和己醛而非丙酮是由体外亚油酸氢过氧化物分解产生的。得出的结论是，与己醛（其通过酶促代谢）和丙酮（其产生受碳水化合物代谢改变等因素主导）相比，烃类气体是更好的脂质过氧化指标。
