Wiegeshaus E H, Harding G, McMurray D, Grover A A, Smith D W
Bull World Health Organ. 1971;45(5):543-50.
The results of research on the immunogenicity of experimental mycobacterial vaccines are characterized by a remarkable lack of agreement about which substances are most immunogenic. The disagreement has usually been attributed to the differences in the methods of preparing the vaccines. An alternative hypothesis is that the conflicting results are a product of the different methods used to assess the potency of the vaccines.To determine if the method by which a vaccine is tested is a major factor contributing to the disagreement, an experiment was conducted in which a series of five different vaccines was distributed to each of nine participating laboratories. Each investigator evaluated the potency of the vaccines in one or more animal models of his own choosing. This in effect held the method of vaccine preparation constant while permitting all other variables to change.The ranking of the five vaccines was random, thus demonstrating that the method by which a vaccine is tested influences the apparent potency of a vaccine. These results cast doubt on the conclusions about the relative potency of tuberculosis vaccines evaluated by different methods.
关于实验性分枝杆菌疫苗免疫原性的研究结果显示，对于哪种物质免疫原性最强，存在明显的不一致。这种不一致通常归因于疫苗制备方法的差异。另一种假设是，相互矛盾的结果是用于评估疫苗效力的不同方法所致。为了确定测试疫苗的方法是否是导致分歧的主要因素，进行了一项实验，将一系列五种不同的疫苗分发给九个参与实验室中的每一个。每位研究者在自己选择的一种或多种动物模型中评估疫苗的效力。这实际上使疫苗制备方法保持不变，同时允许所有其他变量发生变化。五种疫苗的排名是随机的，这表明测试疫苗的方法会影响疫苗的表观效力。这些结果对通过不同方法评估的结核病疫苗相对效力的结论提出了质疑。
