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[乙酰胆碱和细胞外钙离子浓度改变时兔心房动作电位期间的膜电流及钙离子转运]

[Membrane current and Ca++-transport during the action potential of the rabbit atrium changed by acetylcholine and extracellular Ca++-concentration].

作者信息

Nilius B

出版信息

Acta Biol Med Ger. 1979;38(8):1159-73.

PMID:532494
Abstract

Using a modified sucrose gap technique action potential and phase plane trajectories of trabeculae from the left rabbit's auricle were recorded. By means of a numerical treatment and some mathematical model interpretations from these measurements current-voltage-(I-V)-relationships and the amount of inward transported calcium are calculated. A time independent K+-current shows that a voltage region within the anomalous rectification should exist. By acetylcholine the anomalous rectification is abolished and the inward transported amount of Ca-ions by the action potential (Q) decreases. A conductance of the K+-current gK will be dimished by decreasing the extracellular calcium concentration. The amount of inward transported calcium does not change significantly by variation of the extracellular calcium concentration. The calcium amount Q should contribute 17% of the calcium necessary for maximum contraction.

摘要

采用改良蔗糖间隙技术记录了来自兔左心耳小梁的动作电位和相平面轨迹。通过对这些测量数据进行数值处理和一些数学模型解读,计算出电流-电压(I-V)关系以及内向转运的钙量。一个与时间无关的钾电流表明在反常整流中应存在一个电压区域。乙酰胆碱可消除反常整流,并且动作电位介导的钙离子内向转运量(Q)减少。降低细胞外钙浓度会使钾电流的电导gK减小。细胞外钙浓度的变化不会使内向转运的钙量发生显著改变。钙量Q应占最大收缩所需钙量的17%。

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