Siddique I H, Loken K I, Hoyt H H
Appl Microbiol. 1965 Sep;13(5):635-7. doi: 10.1128/am.13.5.635-637.1965.
A study was conducted to design a more sensitive and flexible technique for the assay of neomycin in milk. Sterile Antibiotic Assay medium (Difco; 15 ml) was poured into glass petri dishes with aluminum tops equipped with absorbent discs. Seed agar (4 ml; containing 1% sodium chloride) inoculated with standardized amounts of the test organism (Staphylococcus epidermidis ATCC 12228) was laid over the hardened base agar, and stainless-steel cylinders were placed equal distances apart in the agar. The plates were refrigerated for 30 min and the cups were removed in an atmosphere of minimal contamination. The resultant holes were sealed with melted agar. After preparation, the holes were used for assay procedures. Samples to be assayed were pipetted into the holes and the plates were refrigerated for 90 min at 4 C. Plates were incubated for 18 to 20 hr at 32 C. The zones of inhibition were recorded and compared with a standard curve. The approximate sensitivity of this method was 0.05 mug/ml.
进行了一项研究，以设计一种更灵敏、更灵活的方法来检测牛奶中的新霉素。将无菌抗生素检测培养基（Difco；15毫升）倒入配有铝盖且带有吸水圆盘的玻璃培养皿中。将接种有标准量测试微生物（表皮葡萄球菌ATCC 12228）的种子琼脂（4毫升；含1%氯化钠）铺在凝固的基础琼脂上，并在琼脂中以相等距离放置不锈钢圆柱体。将平板冷藏30分钟，然后在污染最小的环境中取出圆柱体。用融化的琼脂密封形成的孔。制备完成后，这些孔用于检测程序。将待检测的样品移液到孔中，平板在4℃下冷藏90分钟。将平板在32℃下培养18至20小时。记录抑菌圈并与标准曲线进行比较。该方法的近似灵敏度为0.05微克/毫升。
