Zimmerman L
J Bacteriol. 1966 Apr;91(4):1537-42. doi: 10.1128/jb.91.4.1537-1542.1966.
Zimmerman, Leonard (Fort Detrick, Frederick, Md.). Toxicity of copper and ascorbic acid to Serratia marcescens. J. Bacteriol. 91:1537-1542. 1966.-Neutral solutions of ascorbic acid were antibacterial to Serratia marcescens at low but not at high population densities. The toxicity of ascorbate was eliminated by metal-sequestering treatments, and was restored only by the addition of trace amounts of copper salts. Copper-ascorbate was equally toxic under aerobic or anaerobic conditions; its toxicity was abolished by (i) chelating agents that sequestered the copper, (ii) metal-complexing agents that bound to the cells but did not sequester copper, and (iii) iron salts in the presence of air. On the basis of these observations, the toxic effects of copper-ascorbate were attributed to its reaction with vital Fe-containing cellular components.
齐默尔曼，伦纳德（马里兰州弗雷德里克市德特里克堡）。铜和抗坏血酸对粘质沙雷氏菌的毒性。《细菌学杂志》91:1537 - 1542。1966年。——抗坏血酸的中性溶液在低菌量密度时对粘质沙雷氏菌具有抗菌作用，但在高菌量密度时则无此作用。抗坏血酸盐的毒性可通过金属螯合处理消除，且只有添加痕量铜盐才能恢复其毒性。在需氧或厌氧条件下，铜 - 抗坏血酸盐的毒性相同；其毒性可被以下物质消除：（i）螯合铜的螯合剂，（ii）与细胞结合但不螯合铜的金属络合剂，以及（iii）在有空气存在时的铁盐。基于这些观察结果，铜 - 抗坏血酸盐的毒性作用归因于其与细胞内重要含铁成分的反应。
