Li Yan, Pi Qing-Meng, You Hui-Hui, Li Jin-Quan, Wang Peng-Cheng, Yang Xu, Wu Yang
Key Laboratory for Deep Processing of Major Grain and Oil (Wuhan Polytechnic University), Ministry of Education, College of Food Science and Engineering, Wuhan Polytechnic University Wuhan 430023 P. R. China
Department of Plastic and Reconstructive Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine Shanghai 200129 P. R. China.
RSC Adv. 2018 May 18;8(33):18272-18283. doi: 10.1039/c8ra01985a. eCollection 2018 May 17.
A multi-functional anti-pathogen coating with "release-killing", "contact-killing" and "anti-adhesion" properties was prepared from biocompatible polymer encapsulated chlorine dioxide (ClO) which protected the active ingredient from the outside environment. A slow sustained-release of ClO from micelles over fifteen days was detected for long-term release-killing. Micelles only release ClO on demand in minimum inhibitory concentrations. We prepared nanoparticles which were covalently clustered on micelle surfaces to improve contact-killing as well as to improve the stability of the micelle. Copper nanoparticles were generated using the biosynthesis method including l-vitamin C, which avoids the toxicity and allows for the preparation of copper nanoparticles in a green environment. Synergistic anti-pathogen activity could be generated by a combination of micelle released ClO and ascorbic acid. In addition to release-killing and contact-killing, a pluronic polymer coated surface also provides an additional "anti-adhesion" property through its protein-repelling ability. In this research, the designed coating demonstrated a broad-spectrum of activity to kill drug-resistant bacteria, viruses and spores in short period of time. Based on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and anti-oxidase assays, we found that the designed coatings killed the pathogens bio-oxidation. We also carried out acute respiratory toxicity tests in this research. Analysis of blood samples, lung function and histopathological slices indicated that the synthesized micelles allowed a controlled and sustained release of ClO to kill pathogens while maintaining an overall ClO concentration in the air within a safe range.
一种具有“释放杀灭”“接触杀灭”和“抗粘附”特性的多功能抗病原体涂层，是由生物相容性聚合物包裹的二氧化氯（ClO）制备而成，该聚合物可保护活性成分免受外界环境影响。检测到胶束在十五天内缓慢持续释放ClO，以实现长期的释放杀灭效果。胶束仅在最低抑菌浓度下按需释放ClO。我们制备了共价聚集在胶束表面的纳米颗粒，以提高接触杀灭效果并改善胶束的稳定性。使用包括l-维生素C在内的生物合成方法生成铜纳米颗粒，该方法避免了毒性，并允许在绿色环境中制备铜纳米颗粒。胶束释放的ClO与抗坏血酸相结合可产生协同抗病原体活性。除了释放杀灭和接触杀灭外，普朗尼克聚合物涂层表面还通过其蛋白质排斥能力提供额外的“抗粘附”特性。在本研究中，设计的涂层在短时间内展现出对耐药细菌、病毒和孢子的广谱杀灭活性。基于扫描电子显微镜（SEM）、透射电子显微镜（TEM）和抗氧化酶测定，我们发现设计的涂层通过生物氧化作用杀灭病原体。我们还在本研究中进行了急性呼吸道毒性试验。血液样本、肺功能和组织病理学切片分析表明，合成的胶束能够控制并持续释放ClO以杀灭病原体，同时将空气中的ClO总体浓度维持在安全范围内。
