Suzuki K T, Takenaka S, Kubota K
Arch Environ Contam Toxicol. 1979;8(1):85-95. doi: 10.1007/BF01055143.
Metallothioneins with differing Cd/Zn ratios were prepared in vitro from rat liver zinc-thionein by replacing zinc with cadmium and were injected intraperitoneally to female rats. The distribution of cadmium, zinc, and copper in the kidney supernatant fraction was determined using a Sephadex G-75 column. The distribution pattern of cadmium and zinc changed dramatically within 24 hr after the injection. The changes were explained by the degradation and re-synthesis of metallothionein in the kidneys. The necrotic changes of renal tubular lining cells were correlated to the amount of cadmium in the metallothionein but not to the amount of metallothionein (protein).
通过用镉取代锌，在体外从大鼠肝脏锌硫蛋白制备了具有不同镉/锌比例的金属硫蛋白，并将其腹腔注射给雌性大鼠。使用葡聚糖凝胶G - 75柱测定肾脏上清液部分中镉、锌和铜的分布。注射后24小时内，镉和锌的分布模式发生了显著变化。这些变化可以通过肾脏中金属硫蛋白的降解和重新合成来解释。肾小管内衬细胞的坏死变化与金属硫蛋白中的镉含量相关，而与金属硫蛋白（蛋白质）的含量无关。
