在韦荣球菌属、月形单胞菌属和拟杆菌属细菌中，通过琥珀酸的还原羧化作用合成α-酮戊二酸。
Synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in Veillonella, Selenomonas, and Bacteriodes species.
作者信息
Allison M J, Robinson I M, Baetz A L
出版信息
J Bacteriol. 1979 Dec;140(3):980-6. doi: 10.1128/jb.140.3.980-986.1979.
Abstract
Evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. Cultures were grown in media containing unlabeled energy substrates plus [14C]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. Our results indicate alpha-ketoglutarate synthase functions in Selenomonas ruminantium, Veillonella alcalescens, Bacteroides fragilis, Bacteroides vulgatus, Bacteroides uniformis, Bacteroides distasonis, and Bacteroides multiacidus. Evidence for this carboxylation was not found in strains representative of 10 other species.
摘要
在来自瘤胃和其他厌氧生境的厌氧异养生物中寻找琥珀酸还原羧化合成α-酮戊二酸的证据。将培养物在含有未标记能量底物加[14C]琥珀酸的培养基中培养，细胞谷氨酸的合成比细胞天冬氨酸具有更高的比活度被视为α-酮戊二酸合酶活性的证据。我们的结果表明，α-酮戊二酸合酶在反刍月形单胞菌、产碱韦荣球菌、脆弱拟杆菌、普通拟杆菌、单形拟杆菌、解淀粉拟杆菌和多酸拟杆菌中发挥作用。在代表其他10个物种的菌株中未发现这种羧化的证据。
相似文献
1
Synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in Veillonella, Selenomonas, and Bacteriodes species.在韦荣球菌属、月形单胞菌属和拟杆菌属细菌中，通过琥珀酸的还原羧化作用合成α-酮戊二酸。
J Bacteriol. 1979 Dec;140(3):980-6. doi: 10.1128/jb.140.3.980-986.1979.
2
Biosynthesis of alpha-ketoglutarate by the reductive carboxylation of succinate in Bacteroides ruminicola.在反刍拟杆菌中通过琥珀酸的还原羧化作用生物合成α-酮戊二酸。
J Bacteriol. 1970 Oct;104(1):50-6. doi: 10.1128/jb.104.1.50-56.1970.
3
Alpha-ketoglutarate metabolism by cytochrome-containing anaerobes.含细胞色素厌氧菌对α-酮戊二酸的代谢
Can J Microbiol. 1983 Jul;29(7):790-6. doi: 10.1139/m83-128.
4
The influence of extracellular hydrogen on the metabolism of Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium.细胞外氢对反刍类拟杆菌、解脂厌氧弧菌和反刍月形单胞菌代谢的影响。
J Gen Microbiol. 1980 Aug;119(2):485-91. doi: 10.1099/00221287-119-2-485.
5
Alternative pathways for biosynthesis of leucine and other amino acids in Bacteroides ruminicola and Bacteroides fragilis.瘤胃拟杆菌和脆弱拟杆菌中亮氨酸及其他氨基酸生物合成的替代途径。
Appl Environ Microbiol. 1984 Dec;48(6):1111-7. doi: 10.1128/aem.48.6.1111-1117.1984.
6
Propionate formation from cellulose and soluble sugars by combined cultures of Bacteroides succinogenes and Selenomonas ruminantium.产琥珀酸拟杆菌和反刍月形单胞菌联合培养从纤维素和可溶性糖生成丙酸
Appl Microbiol. 1973 Nov;26(5):789-95. doi: 10.1128/am.26.5.789-795.1973.
7
Degradiation of alpha-ketoglutarate by Veillonella alcalescens.产碱韦荣球菌对α-酮戊二酸的降解作用
J Bacteriol. 1969 Apr;98(1):315-6. doi: 10.1128/jb.98.1.315-316.1969.
8
Oxygen metabolism by the anaerobic bacterium veillonella alcalescens.产碱韦荣球菌这种厌氧菌的氧代谢
Arch Microbiol. 1978 Nov 13;119(2):167-74. doi: 10.1007/BF00964269.
9
Utilization of nucleic acids by Selenomonas ruminantium and other ruminal bacteria.反刍月形单胞菌及其他瘤胃细菌对核酸的利用
Appl Environ Microbiol. 1990 Dec;56(12):3867-70. doi: 10.1128/aem.56.12.3867-3870.1990.
10
Rapid presumptive identification of anaerobes in blood cultures by gas-liquid chromatography.通过气液色谱法快速初步鉴定血培养中的厌氧菌。
J Clin Microbiol. 1980 Mar;11(3):274-7. doi: 10.1128/jcm.11.3.274-277.1980.
引用本文的文献
1
New functions of pirin proteins and a 2-ketoglutarate: Ferredoxin oxidoreductase ortholog in Bacteroides fragilis metabolism and their impact on antimicrobial susceptibility to metronidazole and amixicile.脆弱拟杆菌代谢中吡咯啉-5-羧酸还原酶的同系物及 2-酮戊二酸：铁氧还蛋白氧化还原酶对毕赤酵母蛋白新功能的影响及其对甲硝唑和氨曲南药敏性的影响
Microbiologyopen. 2024 Aug;13(4):e1429. doi: 10.1002/mbo3.1429.
2
An enzyme and(13)C-NMR study of carbon metabolism in heliobacteria.一种产甲烷菌碳代谢的酶和（13）C-NMR 研究。
Photosynth Res. 1994 Jul;41(1):75-88. doi: 10.1007/BF02184147.
3
Synthesis of the 1-monoester of 2-ketoalkanedioic acids, for example, octyl α-ketoglutarate.合成 2-酮烷二酸的 1-单酯，例如辛基 α-酮戊二酸酯。
J Org Chem. 2012 Dec 7;77(23):11002-5. doi: 10.1021/jo302308q. Epub 2012 Nov 28.
4
An anaerobic-type alpha-ketoglutarate ferredoxin oxidoreductase completes the oxidative tricarboxylic acid cycle of Mycobacterium tuberculosis.一种厌氧型 α-酮戊二酸铁氧还蛋白氧化还原酶完成结核分枝杆菌的氧化三羧酸循环。
PLoS Pathog. 2009 Nov;5(11):e1000662. doi: 10.1371/journal.ppat.1000662. Epub 2009 Nov 20.
5
Consumption of pasteurized human lysozyme transgenic goats' milk alters serum metabolite profile in young pigs.人溶菌酶转基因山羊奶的消费改变了幼猪的血清代谢物谱。
Transgenic Res. 2010 Aug;19(4):563-74. doi: 10.1007/s11248-009-9334-4. Epub 2009 Oct 22.
6
A mitochondrial-like aconitase in the bacterium Bacteroides fragilis: implications for the evolution of the mitochondrial Krebs cycle.脆弱拟杆菌中一种类似线粒体的乌头酸酶：对线粒体三羧酸循环进化的启示
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4662-7. doi: 10.1073/pnas.052710199. Epub 2002 Mar 5.
7
Whey fermentation by anaerobiospirillum succiniciproducens for production of a succinate-based animal feed additive.琥珀酸厌氧螺菌发酵乳清以生产基于琥珀酸的动物饲料添加剂。
Appl Environ Microbiol. 1999 May;65(5):2260-3. doi: 10.1128/AEM.65.5.2260-2263.1999.
8
The NAD(P)H-utilizing glutamate dehydrogenase of Bacteroides thetaiotaomicron belongs to enzyme family I, and its activity is affected by trans-acting gene(s) positioned downstream of gdhA.多形拟杆菌利用NAD(P)H的谷氨酸脱氢酶属于I类酶家族，其活性受位于gdhA下游的反式作用基因影响。
J Bacteriol. 1996 Dec;178(24):7212-20. doi: 10.1128/jb.178.24.7212-7220.1996.
9
Interconversion of valine and leucine by Clostridium sporogenes.生孢梭菌介导的缬氨酸和亮氨酸的相互转化。
J Bacteriol. 1982 Nov;152(2):946-9. doi: 10.1128/jb.152.2.946-949.1982.
10
Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.采用高效液相色谱法分析厌氧细菌中的短链酸。
J Clin Microbiol. 1982 Aug;16(2):355-60. doi: 10.1128/jcm.16.2.355-360.1982.
本文引用的文献
1
The Mechanism of Propionic Acid Formation by Succinate Decarboxylation: II. The Formation and Decarboxylation of Succinyl-CoA.琥珀酸脱羧形成丙酸的机制：II. 琥珀酰辅酶A的形成与脱羧
Proc Natl Acad Sci U S A. 1953 Aug;39(8):779-85. doi: 10.1073/pnas.39.8.779.
2
Some Nutritional Requirements of the Genus Ruminococcus.瘤胃球菌属的一些营养需求。
Appl Microbiol. 1961 Mar;9(2):91-5. doi: 10.1128/am.9.2.91-95.1961.
3
The anaerobic mesophilic cellulolytic bacteria.厌氧嗜温性纤维素分解菌。
Bacteriol Rev. 1950 Mar;14(1):1-49. doi: 10.1128/br.14.1.1-49.1950.
4
Degradation of pyruvate by Micrococcus lactilyticus. III. Properties and cofactor requirements of the carbon dioxide-exchange reaction.乳酸微球菌对丙酮酸的降解作用。III. 二氧化碳交换反应的特性及辅助因子需求
J Bacteriol. 1963 Feb;85(2):382-93. doi: 10.1128/jb.85.2.382-393.1963.
5
Succinic acid turnover and propionate production in the bovine rumen.牛瘤胃中琥珀酸的周转与丙酸的生成
Appl Microbiol. 1963 Mar;11(2):132-5. doi: 10.1128/am.11.2.132-135.1963.
6
Cytochrome-producing anaerobic Vibrio succinogenes, sp. n.产细胞色素的厌氧琥珀酸弧菌，新种
J Bacteriol. 1961 Jun;81(6):911-7. doi: 10.1128/jb.81.6.911-917.1961.
7
Pathway of propionate formation in Bacteroides ruminicola.瘤胃拟杆菌中丙酸形成途径
J Bacteriol. 1967 Jan;93(1):504-5. doi: 10.1128/jb.93.1.504-505.1967.
8
9
Degradiation of alpha-ketoglutarate by Veillonella alcalescens.产碱韦荣球菌对α-酮戊二酸的降解作用
J Bacteriol. 1969 Apr;98(1):315-6. doi: 10.1128/jb.98.1.315-316.1969.
10
Quantitative collection of 14CO2 in the presence of labeled short-chain acids.在标记的短链酸存在的情况下对¹⁴CO₂进行定量收集。
Anal Biochem. 1969 Feb;27(2):311-4. doi: 10.1016/0003-2697(69)90038-4.
Zotero 插件