采用高效液相色谱法分析厌氧细菌中的短链酸。
Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography.
作者信息
Guerrant G O, Lambert M A, Moss C W
出版信息
J Clin Microbiol. 1982 Aug;16(2):355-60. doi: 10.1128/jcm.16.2.355-360.1982.
Abstract
A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.
摘要
使用Aminex HPX - 87柱，通过高效液相色谱法分离出25种短链脂肪酸的标准混合物。用乙醚萃取后再用少量0.1N NaOH重新萃取，通过高效液相色谱法分析厌氧细菌在培养基中产生的酸。通过对高效液相色谱柱捕获的洗脱液馏分进行气相色谱 - 质谱分析，证实了厌氧消化链球菌培养提取物中存在富马酸。
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