DNA复制的酶促机制。
Enzymatic mechanisms of DNA replication.
作者信息
Mitra S, Kornberg A
出版信息
J Gen Physiol. 1966 Jul;49(6):59-79. doi: 10.1085/jgp.49.6.59.
Abstract
DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching. Aberrations in synthesized DNA were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (M13) DNA served as template. Replication of a single-stranded, circular DNA produced a helical product, but the nature of initiation of a new strand by the circular template remains to be explained. Hypothetical mechanisms for simultaneous replication of the 5'-strand are presented as is the possibility that the tertiary structure of the DNA, as for example, a circular form of the helix, is of prime importance in in vivo replication.
摘要
从多个来源纯化得到的DNA聚合酶的特点是能复制螺旋模板的3'-羟基末端链。未能同时复制5'-链会导致合成DNA出现畸变，表现为不可变性和分支。当（a）在复制过程中5'-链被特定核酸酶破坏，或（b）单链（环状）噬菌体（M13）DNA作为模板时，未观察到合成DNA的畸变。单链环状DNA的复制产生了螺旋产物，但环状模板引发新链的起始机制仍有待解释。文中提出了同时复制5'-链的假设机制，以及DNA的三级结构（如螺旋的环状形式）在体内复制中至关重要的可能性。
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