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DNA复制的酶促机制。

Enzymatic mechanisms of DNA replication.

作者信息

Mitra S, Kornberg A

出版信息

J Gen Physiol. 1966 Jul;49(6):59-79. doi: 10.1085/jgp.49.6.59.

DOI:10.1085/jgp.49.6.59
PMID:5338562
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2195533/
Abstract

DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching. Aberrations in synthesized DNA were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (M13) DNA served as template. Replication of a single-stranded, circular DNA produced a helical product, but the nature of initiation of a new strand by the circular template remains to be explained. Hypothetical mechanisms for simultaneous replication of the 5'-strand are presented as is the possibility that the tertiary structure of the DNA, as for example, a circular form of the helix, is of prime importance in in vivo replication.

摘要

从多个来源纯化得到的DNA聚合酶的特点是能复制螺旋模板的3'-羟基末端链。未能同时复制5'-链会导致合成DNA出现畸变，表现为不可变性和分支。当（a）在复制过程中5'-链被特定核酸酶破坏，或（b）单链（环状）噬菌体（M13）DNA作为模板时，未观察到合成DNA的畸变。单链环状DNA的复制产生了螺旋产物，但环状模板引发新链的起始机制仍有待解释。文中提出了同时复制5'-链的假设机制，以及DNA的三级结构（如螺旋的环状形式）在体内复制中至关重要的可能性。

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本文引用的文献

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AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.一种来自粗糙脉孢菌的核酸内切酶，对缺乏有序结构的多核苷酸具有特异性。I. 酶的纯化及性质
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ENZYMIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XVII. SOME UNUSUAL PHYSICAL PROPERTIES OF THE PRODUCT PRIMED BY NATIVE DNA TEMPLATES.脱氧核糖核酸的酶促合成。十七。由天然DNA模板引发的产物的一些异常物理性质。
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