Comparison of methods for the isolation of Salmonella from bone meal.

The efficiency of some methods for the isolation of Salmonella from bone meal was studied. Grinding of the granulated, industrial product was found to increase Salmonella recovery. An improvement in the efficiency of the pre-enrichment method was obtained by adding deoxycholate (1%) to mannitol broth. Selective enrichment in selenite cystine medium gave better results than in Kauffmann-Müller medium, when small inocula were used. Experimental data showed that, in the presence of large numbers of coliforms and very small numbers of Salmonella, the Kauffman-Müller medium offers better results, but, in the presence of smaller numbers of coliforms, selenite cystine is preferable. As solid selective medium, Brilliant Green Agar gave, in our laboratory, better results than S S medium.