TAYLOR W I, SILLIKER J H
Appl Microbiol. 1961 Nov;9(6):484-6. doi: 10.1128/am.9.6.484-486.1961.
A comparison of various methods of enhancing frequency of Salmonella isolations revealed that inoculation of a second enrichment broth, with culture from the first, was no improvement over the single direct enrichment method. It was inferior to centrifugation. Selenite was observed to produce more positive isolations at 48 hr than at 24. No change occurred in tetrathionate. Reconstitution of dried albumen with water produced a significant increase in isolations over direct inoculation of enrichment broth in the case of tetrathionate but not selenite broth.Pre-enrichment in lactose broth before inoculation of enrichment media was vastly superior to reconstitution in water for both enrichment broths. A comparison of results obtained using dulcitol, mannitol, lactose and carbohydrate-free purple broths in pre-enrichment indicated that the carbohydrate added was immaterial.
对多种提高沙门氏菌分离频率方法的比较表明，用首次培养物接种第二种增菌肉汤，相较于单一直接增菌法并无改进。它不如离心法。观察发现，亚硒酸盐在48小时时产生的阳性分离物比24小时时更多。四硫磺酸盐则无变化。对于四硫磺酸盐肉汤而非亚硒酸盐肉汤，用水复溶干燥蛋白胨后接种增菌肉汤，其分离物数量显著多于直接接种增菌肉汤。在接种增菌培养基之前，在乳糖肉汤中进行预增菌，对于两种增菌肉汤而言，都远优于用水复溶。在预增菌中使用卫矛醇、甘露醇、乳糖和无碳水化合物紫色肉汤所获得结果的比较表明，添加的碳水化合物无关紧要。
