抗原结合性胸腺衍生淋巴细胞。I. 分离θ阳性抗原刺激细胞的快速方法。
Antigen-binding thymus-derived lymphocytes. I. Rapid method for isolation of theta-positive antigen-stimulated cells.
作者信息
Hogg N M, Greaves M F
出版信息
Immunology. 1972 Jun;22(6):959-65.
Abstract
A rapid method for separating SRBC-stimulated populations of theta (θ)-positive thymus-derived lymphocytes from bone marrow-derived lymphocytes and plaque-forming cells has been developed using cotton wool columns. The column effluent was monitored for plaque-forming and `rosette'-forming cells. Stimulated θ-negative cells were found to adhere preferentially to cotton wool. The total recovery of small lymphocytes was 12±3 per cent and the yield of rosette-forming cells was 5.5±4 per cent of which 85±5 per cent were θ-positive.
摘要
已开发出一种快速方法，使用棉纤维柱从骨髓来源的淋巴细胞和形成噬斑细胞中分离出经绵羊红细胞（SRBC）刺激的θ阳性胸腺来源淋巴细胞群体。对柱流出液进行监测，以检测形成噬斑细胞和“花环”形成细胞。发现受刺激的θ阴性细胞优先黏附于棉纤维。小淋巴细胞的总回收率为12±3%，花环形成细胞的产率为5.5±4%，其中85±5%为θ阳性。
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