Kasai M, Kanemasa T, Fukumoto S
J Membr Biol. 1979 Dec 31;51(3-4):311-24. doi: 10.1007/BF01869089.
Osmotic volume change of sarcoplasmic reticulum vesicles was studied by following the change in light-scattering intensity using a stopped flow apparatus. From the analysis of the initial rate of scattering change, reflection coefficients for various ions and neutral molecules were determined. The following are typical results: K+, 0.72; Tris+, 0.98; choline 1; NO3-, 0.32; Cl-, 0.46; methanesulfonate, 0.62; gluconate, 0.96; glycerol, 0.86; and glucose, 1. When the K+ permeability was increased in the presence of 10(-6) g valinomycin/ml, the reflection coefficient for K+ changed from 0.72 to 0.31. It was found that there was a close relationship between the reflection coefficients and the permeabilities of the solutes. Hydraulic conductivity was also determined from the initial rate of light scattering change and was not different for the different solutes. The water permeability was estimated to be 2.1 x 10(-3) cm/sec at 23 degrees C.
利用停流装置跟踪光散射强度的变化，研究了肌浆网囊泡的渗透体积变化。通过分析散射变化的初始速率，确定了各种离子和中性分子的反射系数。以下是典型结果：K +，0.72；Tris +，0.98；胆碱，1；NO3 -，0.32；Cl -，0.46；甲磺酸盐，0.62；葡萄糖酸盐，0.96；甘油，0.86；葡萄糖，1。当在10（-6）g缬氨霉素/毫升存在下K +通透性增加时，K +的反射系数从0.72变为0.31。发现反射系数与溶质的通透性之间存在密切关系。还根据光散射变化的初始速率确定了水力传导率，不同溶质的水力传导率没有差异。在23℃下，水渗透率估计为2.1×10（-3）厘米/秒。
