Varma M G, Pudney M, Leake C J, Peralta P H
Intervirology. 1975;6(1):50-6. doi: 10.1159/000149453.
A simple, rapid and inexpensive method of isolating yellow fever (YF) virus from naturally infected mosquitoes, human liver and the serum of a sentinel monkey by inoculation of a continuous line of mosquito cells is described. The mosquito cells were more sensitive than suckling mice and marginally better than Vero cells for primary isolation. This is the first time that mosquito cells have been successfully used for primary isolation of YF virus from field material.
本文描述了一种通过接种连续传代的蚊细胞，从自然感染的蚊子、人肝脏和哨兵猴血清中分离黄热病毒的简单、快速且廉价的方法。对于初次分离，蚊细胞比乳鼠更敏感，略优于Vero细胞。这是首次成功利用蚊细胞从野外材料中初次分离黄热病毒。
